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User Guides


NanoDrop 1000 User Manual

PDF version of NanoDrop 1000 User Manual available for download.


Nucleic Acid Booklet

A concise, easy-to-use technical resource for Nucleic Acid absorbance measurements using NanoDrop spectrophotometers. This booklet provides quick access to best practice and troubleshooting information.


Colorimetric Assay Booklet

A concise, easy-to-use technical resource for Colorimetric absorbance measurements using NanoDrop spectrophotometers. This booklet provides quick access to best practice and troubleshooting information.


Protein A280 Booklet

A concise, easy-to-use technical resource for A280 absorbance measurements using NanoDrop spectrophotometers. This booklet provides quick access to best practice and troubleshooting information.


Archived Data

Explanation of automatically archived (saved) data.


Blank Measurements

Technical guidance on the use of appropriate blanking solutions.


Sample Reproducibility

General suggestions to ensure accurate and reproducible results when making pedestal measurements.


Nucleic Acid Controls vs. Standards

General discussion of controls vs standards used with the Nucleic Acid application.


Nucleic Acid Purity Ratios

Description of 260/280 and 260/230 ratios as tools for assessing preparation purity. Includes spectral images of common molecular reagents.


Influence of Buffer on Choice of Protein Quantification Method

Technical guidance on the use of appropriate buffer solutions for protein measurements


Nucleic Acid and Protein A280 Baseline Corrections

Explanation of bichromatic baseline correction (normalization) for Nucleic Acid and Protein A280 measurements.


Protein Measurements

Table summary of pre-coded protein assays available in software. Includes method advantages and disadvantages and assay concentration ranges.


Best Practices: Proteins

Recommended practices to achieve optimal results measuring proteins on NanoDrop Instruments. Includes information about common reagents absorbency.


Quick Start Colorimetric Protein Assays

General steps for using pre-coded colorimetric assays. Written in a quick start format for ease of use.


Bradford Assay Tips

Technical guidance to ensure optimal results when using the Bradford Protein Assay on NanoDrop spectrophotometers.


Comparing Microvolume and Cuvette Based Measurements of Microbial Cell Cultures

Helpful hints to optimize microbial cell culture measurements using the pedestal mode. Discusses differences between OD 600 results obtained with a cuvette vs. pedestal.


Solvent Compatibility for NanoDrop products pedestal surfaces

A list of common solvents compatible with the NanoDrop pedestal surfaces. Includes recommendations for the care of the diaphragm surrounding the pedestal of the NanoDrop 2000/2000c.


Dymo Label Writer Setup

Instructions for formatting NanoDrop Instruments for use with the Dymo Label Writer 450 printer.


   
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