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  NanoDrop 1000 Nucleic Acid Concentration and Quality
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Demo Program
 

The Thermo Scientific NanoDropTM 1000 Spectrophotometer will accurately and reproducibly measure nucleic acid samples up to 3700 ng/ul, without dilution. To do this, the instrument automatically detects the high concentration and utilizes the 0.2mm pathlength to calculate the absorbance. A sample measurement output is shown below.
.

Key Features

  • Includes analysis capability for dsDNA, RNA and "other" (analysis constant selectable between 15-55)
  • High absorbance capability: up to 3700 ng/ul for dsDNA
  • Reblank feature allows for the re-analysis of previous sample with a new blank
  • Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
  • 260/280 and 260/230 ratios calculated and displayed
  • Sample ID field is key-focused for easy bar code reader use
  • Absorbance is represented as a 10mm path equivalent

Measurement concentration range

Detection Limit
(ng/ul)
Approximate Upper Limit
(ng/ul)
Typical Reproducibility
(minimum 5 replicates)
(SD= ng/ul; CV= %)
2

3700 (dsDNA)
3000 (RNA)
2400 (ssDNA)

sample range 2-100 ng/ul: ±1.5 ng/ul
sample range >100 ng/ul: ± 2%

 

For more details view the user's manual.

 

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